Bacitracin – 10 μg Antibiotic Disc – Bioanalyse is a paper disc impregnated with 10 micrograms of bacitracin, a cyclic polypeptide antibiotic. It is used in the disc diffusion method of antimicrobial susceptibility testing to determine the susceptibility of bacteria to bacitracin.

The disc diffusion method is a simple and inexpensive method for testing the susceptibility of bacteria to antibiotics. It is based on the principle that bacteria that are susceptible to an antibiotic will not be able to grow near the disc containing the antibiotic.

To perform the disc diffusion test, a bacterial suspension is spread on the surface of an agar plate. Antibiotic discs are then placed on the surface of the agar plate. The plates are incubated for 16-24 hours, and then the zones of inhibition around the discs are measured. The size of the zone of inhibition is proportional to the susceptibility of the bacteria to the antibiotic.

The Bacitracin – 10 μg Antibiotic Disc – Bioanalyse is a valuable tool for the diagnosis and treatment of bacterial infections. It can be used to identify bacteria that are susceptible to bacitracin, and to determine the optimal dose of bacitracin to use for treatment.

Interpretation of results:

• Susceptible: Zone of inhibition is greater than or equal to 23 millimeters.
• Intermediate: Zone of inhibition is 17-22 millimeters.
• Resistant: Zone of inhibition is less than 17 millimeters.

It is important to note that the disc diffusion method is only a semi-quantitative method for testing the susceptibility of bacteria to antibiotics. For more accurate results, other methods such as broth microdilution or agar dilution should be used.

The Bacitracin – 10 μg Antibiotic Disc – Bioanalyse is a valuable tool for the diagnosis and treatment of a variety of bacterial infections, including:

• Skin and soft tissue infections
• Ophthalmic infections
• Otitis media
• Bacterial vaginosis

Limitations of the Bacitracin – 10 μg Antibiotic Disc – Bioanalyse:

• The disc diffusion method is only a semi-quantitative method for testing the susceptibility of bacteria to antibiotics. For more accurate results, other methods such as broth microdilution or agar dilution should be used.
• The disc diffusion method is not able to detect all bacteria that are resistant to bacitracin. For example, some strains of Enterococcus faecium are resistant to bacitracin, but they may not produce a zone of inhibition around a bacitracin disc.
• The disc diffusion method can be affected by the type of agar plate used, the incubation temperature, and the inoculum density. Therefore, it is important to follow standardized procedures when performing the disc diffusion test.

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