Bioindex TSH EIA Kit – 96 Test:

REAGENTS
Materials provided with the kit:

 Goat Anti-Mouse IgG Coated Microtiter Wells, 96 wells
 Enzyme Conjugate Concentrate (11x), 1.3 ml.
 Enzyme Conjugate Diluent, 13 ml.
 T3 Reference Standards, 0, 0.75, 1.5, 3.0, 6.0 and 10.0 ng/ml, 1
set, 1.0 ml each, ready to use.
 Antibody Reagent, 7 ml.
 TMB Reagnet (One-Step), 11 ml.
 Stop Solution (1N HCl), 11 ml

Materials required but not provided:

 Precision pipettes: 50 l, 100 l and 1.0 ml.
 Disposable pipette tips.
 Distilled water.
 Vortex mixer or equivalent.
 Absorbent paper or paper towel.
 Graph paper.
 Microtiter plate reader.

STANDARD CURVE
Concentration
(IU/mL) O.D 450 nm
0 0.051
0.5 0.124
2.0 0.387
5.0 0.862
10.0 1.553
25.0 2.903
This curve was obtained on the date of performance testing of the components. It is for the only
purpose of illustration. Each laboratory should establish its own standard curve