The Cefotaxime – 30 μg Antibiotic Disc – Bioanalyse is a paper disc impregnated with 30 micrograms of cefotaxime, a third-generation cephalosporin antibiotic. It is used in the disc diffusion method of antimicrobial susceptibility testing to determine the susceptibility of bacteria to cefotaxime.

The disc diffusion method is a simple and inexpensive method for testing the susceptibility of bacteria to antibiotics. It is based on the principle that bacteria that are susceptible to an antibiotic will not be able to grow near the disc containing the antibiotic.

To perform the disc diffusion test, a bacterial suspension is spread on the surface of an agar plate. Antibiotic discs are then placed on the surface of the agar plate. The plates are incubated for 16-24 hours, and then the zones of inhibition around the discs are measured. The size of the zone of inhibition is proportional to the susceptibility of the bacteria to the antibiotic.

The Cefotaxime – 30 μg Antibiotic Disc – Bioanalyse is a valuable tool for the diagnosis and treatment of a wide range of bacterial infections, including:

• Community-acquired pneumonia
• Acute otitis media
• Sinusitis
• Urinary tract infections
• Gynecological infections
• Skin and soft tissue infections
• Sepsis
• Meningitis

Interpretation of results:

| Zone of inhibition | Interpretation | |—|—|—| | ≥ 27 mm | Susceptible | | 22-26 mm | Intermediate | | < 22 mm | Resistant |

It is important to note that the disc diffusion method is only a semi-quantitative method for testing the susceptibility of bacteria to antibiotics. For more accurate results, other methods such as broth microdilution or agar dilution should be used.

The Cefotaxime – 30 μg Antibiotic Disc – Bioanalyse is a valuable tool for the diagnosis and treatment of a wide range of bacterial infections. However, it is important to be aware of the potential side effects of cefotaxime before using it. Cefotaxime can cause side effects such as nausea, vomiting, diarrhea, rash, and allergic reactions. In rare cases, cefotaxime can cause serious side effects such as seizures and anaphylaxis.

Limitations of the Cefotaxime – 30 μg Antibiotic Disc – Bioanalyse:

• The disc diffusion method is only a semi-quantitative method for testing the susceptibility of bacteria to antibiotics. For more accurate results, other methods such as broth microdilution or agar dilution should be used.
• The disc diffusion method is not able to detect all bacteria that are resistant to cefotaxime. For example, some strains of Enterobacteriaceae and Pseudomonas aeruginosa are resistant to cefotaxime, but they may not produce a zone of inhibition around a cefotaxime disc.
• The disc diffusion method can be affected by the type of agar plate used, the incubation temperature, and the inoculum density. Therefore, it is important to follow standardized procedures when performing the disc diffusion test.

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