The Doripenem – 10 μg Antibiotic Disc – Bioanalyse is a paper disc impregnated with 10 micrograms of doripenem, a carbapenem antibiotic. It is used in the disc diffusion method of antimicrobial susceptibility testing to determine the susceptibility of bacteria to doripenem.

The disc diffusion method is a simple and inexpensive method for testing the susceptibility of bacteria to antibiotics. It is based on the principle that bacteria that are susceptible to an antibiotic will not be able to grow near the disc containing the antibiotic.

To perform the disc diffusion test, a bacterial suspension is spread on the surface of an agar plate. Antibiotic discs are then placed on the surface of the agar plate. The plates are incubated for 16-24 hours, and then the zones of inhibition around the discs are measured. The size of the zone of inhibition is proportional to the susceptibility of the bacteria to the antibiotic.

The Doripenem – 10 μg Antibiotic Disc – Bioanalyse is a valuable tool for the diagnosis and treatment of a wide range of bacterial infections, including:

• Serious infections caused by multidrug-resistant Gram-negative bacteria, such as carbapenem-resistant Enterobacteriaceae (CRE) and Pseudomonas aeruginosa
• Intra-abdominal infections
• Sepsis
• Meningitis

Interpretation of results:

| Zone of inhibition | Interpretation | |—|—|—| | ≥ 21 mm | Susceptible | | 16-20 mm | Intermediate | | < 16 mm | Resistant |

It is important to note that the disc diffusion method is only a semi-quantitative method for testing the susceptibility of bacteria to antibiotics. For more accurate results, other methods such as broth microdilution or agar dilution should be used.

The Doripenem – 10 μg Antibiotic Disc – Bioanalyse is a valuable tool for the diagnosis and treatment of a wide range of bacterial infections, especially those caused by multidrug-resistant bacteria. However, it is important to be aware of the potential side effects of doripenem before using it. Doripenem can cause side effects such as nausea, vomiting, diarrhea, rash, and allergic reactions. In rare cases, doripenem can cause serious side effects such as seizures and anaphylaxis.

Limitations of the Doripenem – 10 μg Antibiotic Disc – Bioanalyse:

• The disc diffusion method is only a semi-quantitative method for testing the susceptibility of bacteria to antibiotics. For more accurate results, other methods such as broth microdilution or agar dilution should be used.
• The disc diffusion method is not able to detect all bacteria that are resistant to doripenem. For example, some strains of Pseudomonas aeruginosa are resistant to doripenem, but they may not produce a zone of inhibition around a doripenem disc.
• The disc diffusion method can be affected by the type of agar plate used, the incubation temperature, and the inoculum density. Therefore, it is important to follow standardized procedures when performing the disc diffusion test.

Additional information about doripenem:

Doripenem is a carbapenem antibiotic. Carbapenem antibiotics are very effective against a wide range of bacteria, including multidrug-resistant bacteria. However, carbapenem antibiotics can also cause serious side effects, such as seizures and anaphylaxis. Therefore, carbapenem antibiotics should only be used as a last resort for infections caused by multidrug-resistant bacteria.