The Fusidic Acid – 10 μg Antibiotic Disc – Bioanalyse is a paper disc impregnated with 10 micrograms of fusidic acid, a steroidal antibiotic. It is used in the disc diffusion method of antimicrobial susceptibility testing to determine the susceptibility of bacteria to fusidic acid.

The disc diffusion method is a simple and inexpensive method for testing the susceptibility of bacteria to antibiotics. It is based on the principle that bacteria that are susceptible to an antibiotic will not be able to grow near the disc containing the antibiotic.

To perform the disc diffusion test, a bacterial suspension is spread on the surface of an agar plate. Antibiotic discs are then placed on the surface of the agar plate. The plates are incubated for 16-24 hours, and then the zones of inhibition around the discs are measured. The size of the zone of inhibition is proportional to the susceptibility of the bacteria to the antibiotic.

The Fusidic Acid – 10 μg Antibiotic Disc – Bioanalyse is a valuable tool for the diagnosis and treatment of a wide range of bacterial infections, including:

• Skin and soft tissue infections
• Bone and joint infections
• Otitis externa
• Pneumonia
• Sepsis

Interpretation of results:

| Zone of inhibition | Interpretation | |—|—|—| | ≥ 17 mm | Susceptible | | 14-16 mm | Intermediate | | < 14 mm | Resistant |

It is important to note that the disc diffusion method is only a semi-quantitative method for testing the susceptibility of bacteria to antibiotics. For more accurate results, other methods such as broth microdilution or agar dilution should be used.

The Fusidic Acid – 10 μg Antibiotic Disc – Bioanalyse is a valuable tool for the diagnosis and treatment of a wide range of bacterial infections. However, it is important to be aware of the potential side effects of fusidic acid before using it. Fusidic acid can cause side effects such as nausea, vomiting, diarrhea, and rash. In rare cases, fusidic acid can cause serious side effects such as liver damage and jaundice.

Limitations of the Fusidic Acid – 10 μg Antibiotic Disc – Bioanalyse:

• The disc diffusion method is only a semi-quantitative method for testing the susceptibility of bacteria to antibiotics. For more accurate results, other methods such as broth microdilution or agar dilution should be used.
• The disc diffusion method is not able to detect all bacteria that are resistant to fusidic acid. For example, some strains of Staphylococcus aureus are resistant to fusidic acid, but they may not produce a zone of inhibition around a fusidic acid disc.
• The disc diffusion method can be affected by the type of agar plate used, the incubation temperature, and the inoculum density. Therefore, it is important to follow standardized procedures when performing the disc diffusion test.

Additional information about fusidic acid:

Fusidic acid is a steroidal antibiotic. Steroidal antibiotics are effective against a narrow range of bacteria, primarily Gram-positive bacteria. Fusidic acid is a bactericidal antibiotic, which means that it kills bacteria.

Fusidic acid is a safe and effective antibiotic for most people. However, it is important to be aware of the potential side effects of fusidic acid before using it. Fusidic acid is not recommended for people who are allergic to fusidic acid or for people who have liver problems.

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