Nutrient Agar

Nutrient Agar

Our Nutrient Agar is a classic, non-selective solid medium engineered for routine microbiological culture. Its well-balanced blend of peptones (5 g/L), beef/yeast extract (3 g/L), NaCl (5 g/L), and agar (15 g/L) supports reliable microbial growth while maintaining osmotic stability . Simply dissolve 23–28 g/L, sterilize by autoclaving (121 °C, 15 min), cool to around 50 °C, and pour into sterile Petri dishes. The resulting medium is firm, slightly opalescent, and yields vibrant colonies from a broad spectrum of organisms, including E. coli and S. aureus .

For specialized applications, supplements such as defibrinated blood (5–10 % v/v), serum, or growth factors can be added post-autoclave to culture fastidious microbes

Key Features (Bullet Points)

• Versatile General-Purpose Medium: Ideal for cultivating a wide range of non‑fastidious bacteria and fungi

• Balanced Composition: Contains 5 g/L peptone, 3 g/L beef extract (or yeast extract), 15 g/L agar, and 5 g/L NaCl; pH 6.8–7.4

• Easy Preparation: Dissolve 23–28 g of powder in 1 L distilled water, autoclave at 121 °C for 15 min, cool to ~50 °C, and pour plates

• Prepares Firm Plates: Produces clear to slightly opalescent medium that gels at ~45 °C and melts at ~95 °C

• Quality Microbial Growth: Supports strong colony growth of E. coli, S. aureus, Salmonella, Pseudomonas, Enterococcus, and more

• Enrichable Option: Compatible with blood, serum, or other biological supplements for fastidious organisms