Total Bilirubin Complete Kit – Human Biolin

The Total Bilirubin Complete Kit from Human Biolin is a qualitative assay for the determination of total bilirubin in human serum or plasma. The kit uses a colorimetric method to measure the amount of bilirubin in the sample. The results are expressed in milligrams per deciliter (mg/dL).

To perform the assay, a small amount of serum or plasma is added to a cuvette. Reagent A is then added, and the cuvette is mixed. After a few minutes, Reagent B is added, and the cuvette is mixed again. The absorbance of the solution is then measured at a wavelength of 540 nanometers using a spectrophotometer.

The absorbance of the solution is compared to the calibration curve to determine the concentration of bilirubin in the sample.

The Total Bilirubin Complete Kit is a sensitive and accurate assay for the determination of total bilirubin in human serum or plasma. It is used in a variety of clinical settings, including hospitals, clinics, and reference laboratories.

Here are some of the advantages of using the Total Bilirubin Complete Kit:

• It is a sensitive and accurate assay.
• It is easy to use.
• The results are reproducible.
• The kit is available in a variety of formats to meet the needs of different laboratories.

Here are some of the limitations of using the Total Bilirubin Complete Kit:

• It is a qualitative assay, so it cannot be used to quantify the amount of bilirubin in the sample.
• The kit is not suitable for use with all types of samples.
• The results may be affected by certain factors, such as hemolysis and lipemia.

Overall, the Total Bilirubin Complete Kit is a valuable tool for the determination of total bilirubin in human serum or plasma. It is a sensitive and accurate assay that is easy to use and reproducible.

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